Standard C-Fern sowing density is 300 spores/dish (60×15 mm Petri dish). This standard sowing density gives more consistent results with respect to the ratio (or proportion) of male to hermaphroditic gametophytes). At lower densities, proportions of these types can be highly variable. Density can be reduced by adding more water before sowing or by using fewer drops of the spore suspension (1 -3). If fewer drops of the spore suspension are used, substitute drops of plain sterile water to still allow adequate spreading of spores.
