C-Fern sperm can swim for over two hours at 25 °C in the Sperm Release Buffer (SRB) as described in the C-Fern Chemotaxis exercise – Chemical Attraction. (See also the C-Fern Manual for the formulation of SRB.)
Sperm will swim a much shorter time in plain distilled water, although it is more than sufficient to obtain fertilization of eggs by sperm.
