A Method for Observing Ceratopteris richardii SpermHeather Cousino and Jun Tsuji, Siena Heights University, Department of Biology, 1247 E. Siena Heights Drive, Adrian, Michigan 49221-1796, USA ABSTRACT The fern Ceratopteris richardii produces motile sperm that are challenging to observe and study due to their rapid speed. To assess the effectiveness of Protoslo in restricting the […]
DNA Extraction Procedure for Ceratopteris – DNA Preparation AttemptsTim Chipman (July 1995) I have attempted a number of total nucleic acid preps using C. richardii prothalli and have had some degree of success. It appears that the limiting factor in this protocol is the amount of tissue readily available. I have been culturing 2.5L batches […]
DNA Extraction Procedure for Ceratopteris – Modified QIAGEN Genomic-tip ProcedureLeslie G. Hickok 4/96 The following procedure results in yields of about 25 ug per g fresh weight of 2 week old gametophytic tissue. Grind 2-3 g gametophytes in liquid nitrogen with a mortar and pestle. Harvest tissue from ca. 2 week old liquid cultures (50 […]
The Effect of Sucrose Concentration On Sporophyte Development in Ceratopteris Heather Forbes (Instructors: Laurie Brown and Marilyn Garne, West Iredell High School, Statesville NC) ABSTRACT Forbes C-Fern Culture Laboratory at West Iredell High School. Shown above (from left to right) C-Fern Growth Chamber, Sterile Transfer Chamber, and HEPA Air Cleaner. This project involved an in-depth […]
A Method of Producing STS (Sequence Tagged Site) markers from RAPDs (Random Amplified Polymorphic DNAs) in Ceratopteris richardii Cora Chaffin (Provine High School, Jackson, Mississippi) and Robert Hamilton (Mississippi College, Clinton, Mississippi) INTRODUCTION Genes are arranged in specific sequences along chromosomes, with each gene occupying its own particular position (locus) on a chromosome. Together the […]