Overview of Equipment and Supplies

For classroom or independent investigations, the following materials and supplies may be necessary.  Culture Media and Media Preparation (per liter of prepared medium) inorganic salts for Basic C-Fern Nutrient Media OR pre-packaged OR prepared Basic C-Fern Nutrient Medium If using inorganic salts, supplies for weighing and preparing stock solutions (see Basic C-Fern Nutrient Medium recipe) […]

Culture Environment – Temperature, Humidity and Evaporation

An appropriate culture environment helps regulate temperature, humidity, evaporation, and helps give consistent results. Culture Containers Once cultures are inoculated with spores, place them into an appropriate culture container. Culture Dome – consisting of clean plastic greenhouse trays covered with transparent humidity domes. Culture Domes should be thoroughly clean to prevent contamination of cultures by […]

Culture Environment – Lighting

Simple lighting setups can provide adequate illumination for gametophyte growth and can help control culture temperature Figure 1. C-Fern Growth Pod illuminated by a 15-watt screw-in fluorescent bulb placed in a small (ca. 6 in) reflective fixture. Once cultures have been inoculated with spores, place them into an appropriate environment that will provide a constant temperature, 28 […]

Nutrient Medium Preparation – 3 Stock Method

Preparation of C-Fern Basic Nutrient Medium – 3 Stock Solution Method. This method has been modified from the traditional 6-Stock Solution method to make final nutrient solution preparation easier. Preparation of Stock Solutions Materials Macro-, micronutrient and Fe salts (Table 1) Distilled or deionized water 1 L volumetric flask(s) Microbalance Magnetic stir plate and stir […]

Microwave Preparation of C-Fern Powdered Medium

Using a Microwave to Prepare Basic C-Fern Powdered Medium Makes C-Fern Even Easier to Use!C-Fern users who do not have access to an autoclave can successfully use a microwave to prepare Basic C-Fern Powdered Medium. Although the lower temperatures and shorter times of microwaving cannot guarantee sterility to the degree that autoclaving does, this method […]

Culture Establishment – Inoculation or Sowing Spores

Inoculation or “Sowing” of C-Fern Spores on the agar surface. Figure 1. Supplies needed for sowing C-Fern spores Materials Surface sterilized C-Fern spores in a graduated spore vial. (Wild type (RNWT1) and mutant spores are available as pre-sterilized class sized and bulk unsterilized units. Bulk unsterilized spores and spores collected directly from sporophytes should be […]