Magnification is typically not the problem since sperm can be adequately visualized even at a total magnification of only 25 X. Usually, difficulties observing sperm are often associated with improper or insufficient illumination. Bottom (transmitted) illumination is preferred. If adjustments are possible, try increasing or decreasing the intensity of the light source. Also, if a […]
We recommend using Difco-Bacto agar. This is a relatively purified and inexpensive brand that has consistently given excellent results. Other agars, for example, those recommended for plant tissue culture uses or more highly purified types, sometimes yield poor or unusual growth of gametophytes.
For 60-mm diameter Petri dishes approximately 18 milliliters (ml) of nutrient medium should be poured into each dish. One liter (l) of nutrient medium will pour approximately 55 60-mm dishes. Dishes will be approximately 3/4 full.
First, it is important to use Petri dishes that are filled properly. Because C-Fern cultures are maintained for a prolonged period of time it is necessary to fill each dish three-quarters full with Basic C-Fern Nutrient Medium. (See How much medium should I pour into the culture dishes?). Another factor may be the culture environment […]