First, it is important to use Petri dishes that are filled properly. Because C-Fern cultures are maintained for a prolonged period of time it is necessary to fill each dish three-quarters full with Basic C-Fern Nutrient Medium. (See How much medium should I pour into the culture dishes?). Another factor may be the culture environment […]
For 60-mm diameter Petri dishes approximately 18 milliliters (ml) of nutrient medium should be poured into each dish. One liter (l) of nutrient medium will pour approximately 55 60-mm dishes. Dishes will be approximately 3/4 full.