Simple lighting setups can provide adequate illumination for gametophyte growth and can help control culture temperature

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Figure 1. C-Fern Growth Pod illuminated by a 15 watt screw-in fluorescent bulb placed in a small (ca. 6 in) reflective fixture.

Once cultures have been inoculated with spores, place them into an appropriate environmental condition that will provide temperature control and adequate lighting. Lighting source depends on the containers that will hold the cultures.

C-Fern Growth Pod
For each Pod, use continuous illumination by a 15 watt screw-in fluorescent bulb placed in a small (ca. 6") reflective dome fixture. Each C-Fern Growth Pod can accommodate 30 individual 60x15 mm Petri dishes.

C-Fern Culture Dome
Continuous illumination by two 40 W cool white fluorescent tubes at a distance of 45 cm or less from the cultures (about 80 umoles photosynthetically active radiation m-2 sec-1) will accommodate two standard Culture Domes (54 X 27 cm). This will provide enough space for up to 64 individual 60x15 mm Petri dishes. Smaller or larger setups can be used to serve individual needs.

Temperature inside Growth Pods and Culture Dome is more important than light level, so the distance between the Culture Dome and the light source can be varied considerably to obtain a temperature near the optimum.

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Figure 2. C-Fern Culture Dome illuminated 40 W cool white fluorescent tubes in a reflective fixture.